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Bioethics --- Genetic engineering --- Genetic engineering --- CRISPR (Genetics) --- Government policy --- Research

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This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.

Biotechnology. --- Chemical engineering --- Genetic engineering

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This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition is a valuable resource for researchers and scientists who are interested in continuing and/or expanding their knowledge of flow cytometry.

Biotechnology. --- Chemical engineering --- Genetic engineering

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Thermomechanics is a scientific discipline which investigates the behavior of bodies under the action forces and heat input. Thermomechanical phenomena commonly occur in the human environment, from the action of solar radiation to the technological processes. The analysis of these phenomena often requires extensive interdisciplinary knowledge and the application of advanced mathematical apparatus. Thermo-mechanical phenomena are analyzed using analytical and numerical methods. The analytical solution offers a quicker assessment of the searched values and its dependence on the various parameters. Some problems can be solved only by numerical methods, of which the finite element method is commonly used. This book intends to present current trends and methods in solving thermomechanics problems.

Biotechnology. --- Chemical engineering --- Genetic engineering --- Chemical engineering.

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Crops --- Plant biotechnology --- Agricultural biotechnology --- Genetic engineering --- United States.